Mouse Models of Melanoma Bone Metastasis

  • Özge UluçkanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a detailed protocol for the preparation and inoculation of tumor cells intra-cardially and intra-tibially to achieve bone metastasis.

Key words

Bone metastasis Melanoma B16 cells Firefly luciferase Imaging 



I would like to acknowledge Dr. Katherine Weilbaecher, whose lab I learned all these techniques in during my graduate studies. Specific thanks go to my fellow labmates Dr. Angela Hirbe and Dr. Elizabeth Morgan.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Novartis Institutes for Biomedical ResearchBaselSwitzerland

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