In Vivo Models of Rheumatoid Arthritis

  • Bettina Grötsch
  • Aline BozecEmail author
  • Georg Schett
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


Rheumatoid arthritis (RA) models in mice induced by immunization enable the analyses of molecular mechanism involved in disease development, progression, and resolution of inflammation. Here, we describe three different models mimicking clinic symptoms of human rheumatoid arthritis, which could be transferable to any genetically modified mouse. We will present the protocol for collagen-induced arthritis (CIA), K/BxN serum-induced arthritis (SIA), and the antigen-induced arthritis (AIA) models. To do so, we will detail their way of induction but also how to analyze RA clinical score and pathology characterization. Finally, we will briefly discuss the main characteristics and disadvantages of all three models.

Key words

Rheumatoid arthritis Collagen-induced arthritis K/BxN serum-induced arthritis Antigen-induced arthritis 


  1. 1.
    Cojocaru M et al (2010) Extra-articular manifestations in rheumatoid arthritis. Maedica (Buchar) 5(4):286–291Google Scholar
  2. 2.
    Asquith DL et al (2009) Animal models of rheumatoid arthritis. Eur J Immunol 39(8):2040–2044CrossRefGoogle Scholar
  3. 3.
    Christensen AD et al (2016) K/BxN serum-transfer arthritis as a model for human inflammatory arthritis. Front Immunol 7:213CrossRefGoogle Scholar
  4. 4.
    Inglis JJ et al (2007) Collagen-induced arthritis in C57BL/6 mice is associated with a robust and sustained T-cell response to type II collagen. Arthritis Res Ther 9(5):R113CrossRefGoogle Scholar
  5. 5.
    Brand DD, Latham KA, Rosloniec EF (2007) Collagen-induced arthritis. Nat Protoc 2(5):1269–1275CrossRefGoogle Scholar
  6. 6.
    Monach PA, Mathis D, Benoist C (2008) The K/BxN arthritis model. Curr Protoc Immunol Chapter 15: p. Unit 15 22Google Scholar
  7. 7.
    Williams RO (2005) Models of rheumatoid arthritis. Ernst Schering Res Found Workshop 50:89–117CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Medicine 3, Rheumatology and ImmunologyUniversity Hospital ErlangenErlangenGermany

Personalised recommendations