Ex-Vivo Model Systems of Cancer-Bone Cell Interactions
This chapter elaborates on the state-of-the-art experimental procedures utilized in ex-vivo model systems of cancer-bone cell interactions under “static and dynamic” culture conditions and their potential use to understand cellular and molecular mechanisms as well as drug testing and discovery. An additional focus of this chapter is to provide details of how to incorporate varying oxygen tension, viz., hypoxic, normoxic, and hyperoxic, in such studies and regulate the bone biology toward dissociation of the bone remodeling stages to achieve only “bone resorption” or “bone formation” individually.
Key wordsEx-vivo model systems Bone organ cultures Static and dynamic cancer-bone co-cultures Roller-tube model Three-dimensional models Bone resorption and formation
The author acknowledges the support of the Department of Periodontology Henry M. Goldman School of Dental Medicine Boston University.
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