Advertisement

Free Dyadic Social Interaction Test in Mice

  • Ann-Katrin Kraeuter
  • Paul C. Guest
  • Zoltán SarnyaiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1916)

Abstract

Psychiatric disorders affect approximately one quarter of people worldwide at some point in their lifetime. This chapter provides a step-by-step guide to conduct behavioral tests in adult mice for investigations of social behavior, without the need for specific equipment. This test should allow the identification of key abnormalities in social interactions that can be followed up by targeted, more complex, behavioral analysis aimed at identification of new biomarkers and potential drug targets.

Key words

Behaviour Mice Social behavior Social interaction Biomarkers Drug targets 

References

  1. 1.
    American Psychiatric Association (2013) Diagnostic and statistical manual of mental disorders, fifth edition (DSM-5), 5th edn. American Psychiatric Publishing, Washington, DC (31 May 2013). ISBN-10: 0890425558CrossRefGoogle Scholar
  2. 2.
    McArthur RA, Borsini F (eds) (2008) Animal and translational models for CNS drug discovery: psychiatric disorders. Academic Press, New York (20 Nov. 2008). ISBN-10: 0123738563Google Scholar
  3. 3.
  4. 4.
    Kluge W, Alsaif M, Guest PC, Schwarz E, Bahn S (2011) Translating potential biomarker candidates for schizophrenia and depression to animal models of psychiatric disorders. Expert Rev Mol Diagn 11(7):721–733CrossRefGoogle Scholar
  5. 5.
    Sarnyai Z, Alsaif M, Bahn S, Ernst A, Guest PC, Hradetzky E et al (2011) Behavioral and molecular biomarkers in translational animal models for neuropsychiatric disorders. Int Rev Neurobiol 101:203–328CrossRefGoogle Scholar
  6. 6.
    File SE (1980) The use of social interaction as a method for detecting anxiolytic activity of chlordiazepoxide-like drugs. J Neurosci Methods 2:219–238CrossRefGoogle Scholar
  7. 7.
    Lapin IP, Mutovkina LG, Ryzov IV, Mirzaev S (1996) Anxiogenic activity of quinolinic acid and kynurenine in the social interaction test in mice. J Psychopharmacol 10(3):246–249CrossRefGoogle Scholar
  8. 8.
    File SE, Seth P (2003) A review of 25 years of the social interaction test. Eur J Pharmacol 463:35–53CrossRefGoogle Scholar
  9. 9.
    Navarro JF, Rivera A, Maldonado E, Cavas M, de la Calle A (2004) Anxiogenic-like activity of 3,4-methylenedioxy-methamphetamine (“Ecstasy”) in the social interaction test is accompanied by an increase of c-fos expression in mice amygdala. Prog Neuro-Psychopharmacol Biol Psychiatry 28(2):249–254CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ann-Katrin Kraeuter
    • 1
    • 2
  • Paul C. Guest
    • 3
  • Zoltán Sarnyai
    • 1
    • 2
    Email author
  1. 1.Laboratory of Psychiatric Neuroscience, Australian Institute of Tropical Health and MedicineJames Cook UniversityTownsvilleAustralia
  2. 2.Discipline of Biomedicine, College of Public Health, Medicine and Veterinary SciencesJames Cook UniversityTownsvilleAustralia
  3. 3.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of BiologyUniversity of Campinas (UNICAMP)CampinasBrazil

Personalised recommendations