The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents

  • Ann-Katrin Kraeuter
  • Paul C. Guest
  • Zoltán SarnyaiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1916)


The elevated plus maze test is used to measure anxiety-like behavior in rodents. It can be used to gain insight into conditions such as posttraumatic stress disorder (PTSD) and other conditions marked by anxious behavior. It can also be used as a component in screening of novel compounds for anxiolytic properties. This model is based on aversion to open spaces, which is seen as the animal spending more time in the enclosed arms of the maze. This chapter describes the steps necessary for setting up and conducting the test, along with interpretation of the results.

Key words

Behavior Anxiety Rodent Mouse Elevated plus maze Anxiolytic 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ann-Katrin Kraeuter
    • 1
    • 2
  • Paul C. Guest
    • 3
  • Zoltán Sarnyai
    • 1
    • 2
    Email author
  1. 1.Laboratory of Psychiatric Neuroscience, Australian Institute of Tropical Health and MedicineJames Cook UniversityTownsvilleAustralia
  2. 2.Discipline of Biomedicine, College of Public Health, Medicine and Veterinary SciencesJames Cook UniversityTownsvilleAustralia
  3. 3.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of BiologyUniversity of Campinas (UNICAMP)CampinasBrazil

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