A Rat Eye Lens Model of Cataract Formation
This chapter describes the use of lenses obtained from rats as a model of cataractogenesis. At the molecular level, this is visualized as reduced activity of oxidative reductive enzymes such as aldose reductase and increased proteolysis of lens structural proteins including vimentin. In this chapter, protocols for assessment of these two pathways are presented. Specifically, this analysis shows a comparison of aldose reductase activity and vimentin cleavage in male and female rat lenses. This is because female rats are more susceptible to cataract formation compared to males.
Key wordsDiabetes Drug treatment Eye lens Aldose reductase Vimentin Proteolysis
- 9.Babizhayev MA (2011) Mitochondria induce oxidative stress, generation of reactive oxygen species and redox state unbalance of the eye lens leading to human cataract formation: disruption of redox lens organization by phospholipid hydroperoxides as a common basis for cataract disease. Cell Biochem Funct 29:183–206CrossRefPubMedGoogle Scholar
- 10.Babizhayev MA, Yegorov YE (2016) Reactive oxygen species and the aging eye: specific role of metabolically active mitochondria in maintaining lens function and in the initiation of the oxidation-induced maturity onset cataract—a novel platform of mitochondria-targeted antioxidants with broad therapeutic potential for redox regulation and detoxification of oxidants in eye diseases. Am J Ther 23:e98–e117. https://doi.org/10.1097/MJT.0b013e3181ea31ffCrossRefPubMedGoogle Scholar