Monosodium Glutamate (MSG)-Induced Animal Model of Type 2 Diabetes

  • Zahra Bahadoran
  • Parvin Mirmiran
  • Asghar GhasemiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1916)


In 1976, an animal model of type 2 diabetes (T2DM) was described by Cameron et al. using injection of monosodium glutamate (MSG) in KK mice during the neonatal period. Some years later, similar models have been developed by various doses and durations and the main of these models exhibited obesity and features of diabetes mellitus, including glycosuria, hyperglycemia, hyperinsulinemia, decreased glucose tolerance, and insulin sensitivity. Studies indicated that MSG treatment of newborn animals generates necrosis of neuronal cells of the hypothalamic ventromedial nucleus and arcuate nucleus. Neonatal MSG-treatment was related to normoglycemic-normoinsulinemic state at young ages and development of obesity and hyperinsulinemia at adult ages. Following observation of a severe hypertrophy of pancreatic islets due to the proliferation of β-cells in MSG-treated mice, this model has been proposed as a useful animal model of human T2DM. A higher dose of MSG (≥4 mg/g body weight) accompanied by a longer follow-up duration (>6 months) are needed to establish a typical animal model of T2DM.

Key words

Monosodium glutamate Type 2 diabetes Obesity Insulin resistance Animal model 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zahra Bahadoran
    • 1
  • Parvin Mirmiran
    • 1
  • Asghar Ghasemi
    • 2
    Email author
  1. 1.Nutrition and Endocrine Research Center, Research Institute for Endocrine SciencesShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Endocrine Physiology Research Center, Research Institute for Endocrine SciencesShahid Beheshti University of Medical SciencesTehranIran

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