Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes

  • Paul C. Guest
Part of the Methods in Molecular Biology book series (MIMB, volume 1916)


The Goto-Kakizaki (GK) rat is a model of type 2 diabetes mellitus, produced originally by selective inbreeding for a hyperglycemic trait. These rats are characterized as having insulin resistance and an insulin secretory defect. Pancreatic islets from these mice show abnormal morphology in the distribution of glucagon-secreting α cells and insulin-secreting β cells, which may affect paracrine functions involved in the secretory response. This chapter describes the characterization of GK rat islets using immunofluorescence microscopy and Western blot analyses to demonstrate the effects on islet architecture and how this translates to changes in expression of predominant islet proteins.

Key words

Goto-Kakizaki rat Type 2 diabetes Insulin Secretion Glucagon Islets of Langerhans Morphology 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Paul C. Guest
    • 1
  1. 1.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of BiologyUniversity of Campinas (UNICAMP)CampinasBrazil

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