Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9
Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.
Key wordsPhotoperiod-sensitive genic male sterility (PGMS) Two-line hybrid rice Carbon starved anther (csa) CRISPR-Cas9 Agrobacterium-mediated transformation Transgene-free
This work was supported by the funds from the National Transgenic Major Program (2016ZX08009003-003-007) and National Key Research and Development Program of China (2016YFD0101107) to ZY.
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