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Analysis of Tissue-Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry

  • Felipe Galvez-Cancino
  • Ernesto Lopez
  • Alvaro LladserEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1913)

Abstract

The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8+ T cells by multi-parametric flow cytometry.

Key words

Flow cytometry Barrier tissues Immunophenotyping Memory CD8+ T cells Single-cell suspensions 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Felipe Galvez-Cancino
    • 1
  • Ernesto Lopez
    • 1
  • Alvaro Lladser
    • 1
    Email author
  1. 1.Laboratory of Gene ImmunotherapyFundación Ciencia & VidaSantiagoChile

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