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Direct Ex Vivo Functional Analysis of HCV-Specific T Cells

  • Su-Hyung Park
  • Eui-Cheol ShinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1911)

Abstract

CD8+ and CD4+ T cells play a critical role in viral clearance during self-limited HCV infection. Moreover, induction of robust and polyfunctional memory T cells is a primary goal in the development of T-cell-based HCV prophylactic vaccines. Direct ex vivo function of HCV-specific T cells without in vitro expansion can be assessed by measuring cytokine secretion or cell proliferation upon in vitro stimulation with HCV antigens using enzyme-linked immunospot (ELISpot) assays, intracellular cytokine staining (ICS) assays, and flow cytometry-based T-cell proliferation assays. In particular, polyfunctionality of T cells can be assessed by ICS assays using multicolor flow cytometry. Here, we provide protocols to measure direct ex vivo functions of HCV-specific T cells.

Key words

Hepatitis C virus T cells Cytokines Proliferation Polyfunction Flow cytometry 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Translational Immunology and VaccinologyGraduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea
  2. 2.Laboratory of Immunology and Infectious DiseasesGraduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea

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