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Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy

  • Pedro Matos Pereira
  • Caron Jacobs
  • Joe GroveEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1911)

Abstract

Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.

Key words

Microscopy Super-resolution Imaging Virus Hepatitis 

Notes

Acknowledgments

We would like to thank Ricardo Henriques and Lucas Walker for advice and technical support. JG is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (107653/Z/15/Z). PMP is supported by a Biotechnology and Biological Sciences Research Council grant (BB/M022374/1). CJ is a Commonwealth Scholar, funded by the UK government. The imaging systems used in this work were funded by the Medical Research Council (MR/K015826/1).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental BiologyUniversity College LondonLondonUK
  2. 2.Division of Infection and Immunity, Institute of Immunity and TransplantationUniversity College LondonLondonUK

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