Application of Deep Mutational Scanning in Hepatitis C Virus
Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.
Key wordsHCV Deep mutational scanning Mutagenesis Next-generation sequencing Viral fitness
NCW was supported by the Croucher Foundation Fellowship.