Analysis for the Heterogeneity of Liver Progenitor Cells

  • Kenji KamimotoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)


Recent technological advances have revealed the heterogeneity of cells and tissues. Existence of heterogeneity in hepatic progenitor cells is becoming apparent by various experimental approaches, and here we describe a series of techniques to investigate the proliferative heterogeneity of these cells. We have developed a new technique by combining genetic lineage tracking and three-dimensional imaging methods. The data obtained can be used in statistical analysis to quantitatively investigate the mechanisms underlying the heterogeneity of hepatic progenitor cells.

Key words

Heterogeneity Cell proliferation Three-dimensional imaging Microscopy Genealogy tracking Quantitative analysis 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Developmental BiologyWashington University School of Medicine in St. LouisSt. LouisUSA

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