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Isolation and Expansion of Rat Hepatocytic Progenitor Cells

  • Junichi Kino
  • Norihisa Ichinohe
  • Masayuki Ishii
  • Toshihiro Mitaka
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Abstract

This protocol showed procedures to isolate and expand small hepatocytes (SHs), as hepatocytic progenitor cells, from a rat liver. SHs are identified as a subpopulation of mature hepatocytes in a healthy liver. SHs can proliferate to form colonies in serum-free medium on hyaluronic acid-coated dishes, of which cells show CD44 positivity (CD44+ SHs). CD44+ SHs can be separated and purified from colonies by using anti-CD44 antibodies after enzymatic dissociation. CD44+ SHs can proliferate to form colonies on Engelbreth-Holm-Swarm gel (EHS-gel)-coated dishes in the serum-free medium for a long period and subculture for several times. Even after the second passage, the cells possess characteristics of hepatocytes such as expression of albumin and HNF4α. In addition, when the cells are treated with EHS-gel, they can recover highly differentiated functions of hepatocytes such as glycogen production, CYP activity, and bile secretion.

Key words

Small hepatocyte Serum-free culture CD44 Hepatocytic function 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Junichi Kino
    • 1
    • 2
  • Norihisa Ichinohe
    • 1
  • Masayuki Ishii
    • 1
    • 3
  • Toshihiro Mitaka
    • 1
  1. 1.Department of Tissue Development and RegenerationResearch Institute for Frontier Medicine, Sapporo Medical University School of MedicineSapporoJapan
  2. 2.Tokushima Research Institute, Otsuka Pharmaceutical Co. Ltd.TokushimaJapan
  3. 3.Department of Surgery, Surgical Oncology and ScienceSapporo Medical University School of MedicineSapporoJapan

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