Identification and Isolation of Clonogenic Cholangiocyte in Mouse

  • Bin LiEmail author
  • Craig Dorrell
  • Pamela S. Canady
  • Leslie Wakefield
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)


Cholangiocytes are proliferative and are one of the sources for liver progenitor cells. Clonogenic cholangiocytes are defined as cells capable of clonally proliferating and differentiating cholangiocytes both in vitro and in vivo. In this protocol, we describe the method for isolation of primary cholangiocytes from mouse. To study the heterogeneity of cholangiocytes, we used flow cytometry-based cell sorting to isolate different subsets of cholangiocytes. Organoid-forming efficiencies from sorted single cells are compared within different cholangiocyte populations to identify clonogenic cholangiocytes.

Key words

Duct cells ST14 FACS Organoid 



The authors thank members in the Markus Grompe Lab at Oregon Health and Science University for technical supports. The authors also thank Oregon Health and Science University Flow Cytometry Core for technical assistance.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Bin Li
    • 1
    Email author
  • Craig Dorrell
    • 1
  • Pamela S. Canady
    • 1
  • Leslie Wakefield
    • 1
  1. 1.Oregon Stem Cell Center, Oregon Health and Science UniversityPortlandUSA

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