A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells

  • Sei KakinumaEmail author
  • Akihide Kamiya
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)


Hepatic progenitor cells are defined as cells exhibiting potency for active proliferation and capacity for bipotential differentiation into hepatocytes and cholangiocytes. To prove the capacity of target cells for terminal differentiation and reconstitution of organs, cell transplantation models have been widely used in previous studies, including those involving the liver. Here we describe a protocol for transplantation of hepatic progenitor cells using retrorsine pretreatment and partial hepatectomy. This transplantation assay reveals the potential for reconstitution of hepatocytes in recipient livers by primary hepatic progenitor cells. Donor cells are detected as a colony composed of 5–10 mature hepatocytes.

Key words

Hepatoblasts Hepatocytes Intrasplenic injection Partial hepatectomy Retrorsine Transplantation Differentiation 



This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology in Japan, Japan Agency for Medical Research and Development, and the Japanese Society of Gastroenterology. We thank Prof. H. Nakauchi (the University of Tokyo), Prof. A. Miyajima (the University of Tokyo), and Dr. N. Tanimizu (Sapporo Medical University) for the helpful discussion.


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Authors and Affiliations

  1. 1.Department of Gastroenterology and HepatologyTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Department of Liver Disease ControlTokyo Medical and Dental UniversityTokyoJapan
  3. 3.Department of Molecular Life SciencesTokai University School of MedicineIseharaJapan

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