Culture System of Bile Duct-Like Cystic Structures Derived from Human-Inducible Pluripotent Stem Cells

  • Akihide KamiyaEmail author
  • Kazuya Anzai
  • Kota Tsuruya
  • Hiromi Chikada
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)


Inducible pluripotent stem (iPS) cells are multipotent stem cells that are produced by gene transfer of reprogramming factors to somatic cells. They are thought to be an important source of regenerative medicine because of their pluripotency and self-renewal ability. Although the liver has high regeneration ability, continuous death of hepatocytes due to chronic inflammation leads to liver cirrhosis and liver carcinoma. With regard to such serious liver diseases, liver transplantation is used as a complete cure, but there is a problem of donor shortage. Therefore, transplantation therapy using liver tissue generated from stem cells in vitro is expected.

We are developing a system to induce the differentiation of cholangiocytes, one of important non-parenchymal cells in living liver tissue, from human iPS cells. Bile duct-like cystic structures can be induced by purifying human iPS cell-derived hepatoblasts expressing hepatic progenitor cell surface markers and inducing differentiation under appropriate culture conditions. These cells are considered to be useful in constructing a hepatic organoid that reproduces the liver structure of the living body.

Key words

Hepatic progenitor cells Human iPS cells Induction of hepatic differentiation Long-term proliferation Purification of progenitor cell 



This work was supported in part by the Education and Research Support Center of Tokai University. Some figures have been reproduced from [12]. This work was supported by a Grant-in-Aid for JSPS Fellows and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Akihide Kamiya
    • 1
    • 2
    Email author
  • Kazuya Anzai
    • 3
  • Kota Tsuruya
    • 3
  • Hiromi Chikada
    • 1
  1. 1.Department of Molecular Life SciencesTokai University School of MedicineIseharaJapan
  2. 2.Center for Matrix Biology and Medicine, Graduate Scool of MedicineTokai UniversityIseharaJapan
  3. 3.Department of GastroenterologyTokai University School of MedicineIseharaJapan

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