A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning
Panning is a common process used for antibody selection from phage antibody libraries. There are several methods developed for a similar purpose, namely streptavidin mass spectrometry immunoassay (MSIA™) Disposable Automation Research Tips, magnetic beads, polystyrene immunotubes, and microtiter plate. The advantage of using a magnetic particle processor system is the ability to carry out phage display panning against multiple target antigens simultaneously in parallel. The system carries out the panning procedure using magnetic nanoparticles in microtiter plates. The entire incubation, wash, and elution process is then automated in this setup. The system also allows customization for the introduction of different panning stringencies. The nature of the biopanning process coupled with the limitation of the system means that minimal human intervention is required for the infection and phage packaging stage. However, the process still allows for rapid and reproducible antibody generation to be carried out.
Key wordsPanning Antibody library Monoclonal antibodies Phage display Semi-automated Magnetic nanoparticles
The authors would like to acknowledge the support from the Malaysian Ministry of Education under the Higher Institution Centre of Excellence (HICoE) Grant (Grant no. 311/CIPPM/44001005).
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