Screening of Transgenic Cotton Based on a Porous Silicon Biosensor
Label-free porous silicon (PSi)-based biosensor enables the detection and identification of the protein in transgenic cotton. Changes in optical response signal in the presence of the target protein can be detected by Fourier transform infrared (FTIR) spectromicroscopy when binding of the target protein with its antibody is selectively captured on the PSi biosensor. Here we describe the transgenic protein (antifreeze protein, AFP), and compare with non-transgenic plants. Significant red shifts are observed for transgenic antifreeze protein cotton lines.
Key wordsTransgenic cotton Antibody Porous silicon Protein biosensor
This work was supported by NSFC grant (No. 31060292) and a key grant from the Ministry of Agriculture of China (No. 2009ZX08005-022B).
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