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Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay

  • Hanna Davideit
  • Annekathrin Haberland
  • Sabine Bartel
  • Sarah Schulze-Rothe
  • Johannes Müller
  • Katrin WenzelEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)

Abstract

Agonistically acting autoantibodies directed against the adrenergic beta-1 receptor (beta1-AABs) are a pathogenic factor in diseases of the heart and circulatory system such as dilated cardiomyopathy. Here we describe the detection of such functionally active beta1-AABs from serum samples using spontaneously beating neonatal rat cardiomyocytes, which express the fully functional adrenergic beta-1 receptor coupled with the signal transduction pathway that induces chronotropy. With serum samples added (containing beta1-AABs), an increased positive chronotropic effect is caused that can be blocked by the subsequent addition of specific beta-blockers (abolishing this chronotropic response). The return to the basal beat rate of the cells by the addition of a beta-blocker proves the adrenergic beta-1 receptor specificity of the serum sample.

Key words

Autoantibodies Adrenergic beta-1 receptor Beta-1 adrenoceptor Bioassay DCM Dilated cardiomyopathy Spontaneously beating neonatal rat cardiomyocytes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hanna Davideit
    • 1
  • Annekathrin Haberland
    • 1
  • Sabine Bartel
    • 1
  • Sarah Schulze-Rothe
    • 1
  • Johannes Müller
    • 1
  • Katrin Wenzel
    • 1
    Email author
  1. 1.Berlin Cures GmbHBerlinGermany

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