Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry

  • Satoshi YamashitaEmail author
  • Nozomu Tawara
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)


Sporadic inclusion body myositis (sIBM) is a chronic and progressive inflammatory myopathy that is the commonest among population over 50s. Recently, autoantibodies against cytosolic 5′-nucleotidase 1A (cN1A) have been identified in plasma and serum samples from patients with sIBM. So far, various methods have been established to detect the anti-cN1A autoantibodies, which showed a clinical utility of detection of the autoantibodies in the diagnosis of sIBM. Here we describe a novel cell-based assay for detection of the autoantibodies, which seems equivalent in sensitivity and better in specificity for the diagnosis of sIBM, compared with the previous methods.

Key words

Cytosolic 5′-nucleotidase 1A (cN1A) Cell-based assay Sporadic inclusion body myositis Autoantibodies Autoantigens 



This work was supported by a Grant-in-Aid for Scientific Research (24591269) from the Japan Society for the Promotion of Science (JSPS), Japan; and a Grant-in-Aid for Research on Intractable Diseases from the Ministry of Health, Labour, and Welfare of Japan. We thank all members of the Department of Diagnostic Medicine and the Department of Neurology, and of Kumamoto University Hospital, for biochemical and histological analyses.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Neurology, Graduate School of Medical SciencesKumamoto UniversityKumamotoJapan

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