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Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry

  • Jehan Alam
  • Sumin Jeon
  • Youngnim ChoiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)

Abstract

Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.

Key words

Aquaporin 5 Autoantibodies Immunofluorescence cytochemistry Cell-based assay Plasma membrane 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Dental Research Institute, School of DentistrySeoul National UniversitySeoulRepublic of Korea
  2. 2.Mary and Dick Holland Regenerative Medicine ProgramUniversity of Nebraska Medical CenterOmahaUSA

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