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Nonspecific Binding in Immunoassays for Autoantibodies

  • Gunnar HouenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)

Abstract

Immunoassays are invaluable for detection and quantification of numerous analytes, including autoantibodies. However, human sera often yield high nonspecific binding in such assays, and this may result in false positive or sometimes false negative results. The causes of nonspecific binding are numerous and it correlates with inflammatory parameters. Since the results of autoantibody testing are used for diagnosis and treatment of autoimmune diseases, it is mandatory to be aware of all possible causes of nonspecific binding for each individual assay and to correct for it whenever possible. General guidelines for this are described in this chapter.

Key words

Autoantibodies Immunoassay Immunocytochemistry Immunohistochemistry False positive False negative Nonspecific binding Positive predictive value Negative predictive value Sensitivity Specificity 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of AutoimmunologyStatens Serum InstitutCopenhagenDenmark
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of Southern DenmarkOdenseDenmark

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