Autoantibodies pp 205-219 | Cite as

Determination of CRP Autoantibodies by SPR Immunoassay

  • Qiu-Yu Li
  • Hai-Yun Li
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)


Biosensors based on the principle of surface plasmon resonance (SPR) are surface-sensitive optical devices used for monitoring biomolecular interactions at the sensor surface in real time without any labeling. It is used in a wide variety of areas including proteomics, clinical diagnosis, environmental monitoring, drug discovery, and food analysis. C-reactive protein (CRP) is a marker of inflammation, which undergoes conformation changes in local lesions, leading to the formation of mCRP. Autoantibodies against mCRP are frequently detected in systemic lupus erythematosus (SLE) and associated with disease activity and prognosis. An SPR immunoassay for CRP autoantibodies at complement factor H–CRP interface is described in this chapter.

Key words

Surface plasmon resonance CRP Complement factor H CRP autoantibody Immobilization 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Qiu-Yu Li
    • 1
  • Hai-Yun Li
    • 2
  1. 1.Department of Respiratory and Critical Care MedicinePeking University Third HospitalBeijingPeople’s Republic of China
  2. 2.MOE Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anPeople’s Republic of China

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