Autoantibodies pp 197-203 | Cite as

Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay

  • Zhiyuan Zhao
  • Yong Gu
  • Dongmei Miao
  • Eric Hoffmeyer
  • Yu Liu
  • Liping Yu
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)


Appearance of autoantibodies to tissue transglutaminase (TGA) is the most reliable biomarker to identify celiac disease autoimmunity. A nonradioactive assay of determination of TGA was newly developed using electrochemiluminescence (ECL) technology. This ECL assay has been demonstrated to be more sensitive than current standard radio-binding assay (RBA) in detecting TGA and can detect TGA earlier among high-risk young children followed from birth.

Key words

Celiac disease Tissue transglutaminase Autoantibodies ECL assay 



This study was supported by JDRF grant 2-SRA-2015-51-Q-R, NIH grant DK32083 and DK32493.

Disclosures The authors have nothing to disclose.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhiyuan Zhao
    • 1
    • 2
  • Yong Gu
    • 1
    • 3
  • Dongmei Miao
    • 1
  • Eric Hoffmeyer
    • 1
  • Yu Liu
    • 2
  • Liping Yu
    • 1
  1. 1.Barbara Davis Center for Childhood DiabetesUniversity of ColoradoAuroraUSA
  2. 2.Department of EndocrinologyThe Second Hospital of Jilin UniversityJilinChina
  3. 3.Department of EndocrinologyFirst Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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