Analysis of Anti-C1q Autoantibodies by Western Blot
Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Results may help clinicians to differentiate between SLE and HUVS.
Key wordsAnti-C1q autoantibodies Western blot C1q protein chains B and C Systemic lupus erythematosus Hypocomplementemic urticarial vasculitis syndrome
We wish to acknowledge the work of those who, together with Lennart Truedsson, first published the use of Western blot analysis of anti-C1q antibodies ; Ulla Mårtensson, the late Anders Sjöholm, and the late Anna-Brita Laurell.
- 17.Sjöwall C, Mandl T, Skattum L, Olsson M, Mohammad AJ (2018). Epidemiology of hypocomplementaemic urticarial vasculitis (anti-C1q vasculitis). Rheumatology(Oxford) 57(8):1400–1407Google Scholar