Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

  • Mariana PiuriEmail author
  • Graham F. Hatfull
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)


Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.

Key words

Reporter phage Mycobacteria DST Fluoromycobacteriophage Antibiotics Resistance 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Química Biológica, Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos Aires, IQUIBICEN-CONICETBuenos AiresArgentina
  2. 2.Laboratorio “Bacteriófagos y Aplicaciones Biotecnológicas”, Departamento de Química Biológica, FCEyN, UBACiudad UniversitariaCiudad Autónoma de Buenos AiresArgentina
  3. 3.Department of Biological Sciences, Pittsburgh Bacteriophage InstituteUniversity of PittsburghPittsburghUSA

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