Bacteriophages pp 173-181 | Cite as

Use of a Silkworm Larva Model in Phage Therapy Experiments

  • Jumpei Uchiyama
  • Iyo Takemura-Uchiyama
  • Shigenobu MatsuzakiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)


Antibiotic-resistant bacteria can cause intractable infections in humans and animals, with damaging effects to health care and economics. Phage therapy is considered a possible alternative to chemotherapy for treating infections, but still requires laborious in vivo experiments before its introduction into society and its further development. Recently, silkworm larvae have been recognized as highly convenient and useful model animals, and an alternative to higher animals. We describe the procedure for experimental phage therapy to treat Staphylococcus aureus infections in silkworm larvae.

Key words

Animal model Phage purification Phage therapy Silkworm larvae Staphylococcus aureus 



We would like to thank Dr. Masanori Daibata and Ms. Takako Ujihara, Kochi University for support with the experiments.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jumpei Uchiyama
    • 1
  • Iyo Takemura-Uchiyama
    • 1
  • Shigenobu Matsuzaki
    • 2
    Email author
  1. 1.School of Veterinary MedicineAzabu UniversityKanagawaJapan
  2. 2.Department of Microbiology and Infection, Kochi Medical SchoolKochi UniversityKochiJapan

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