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Identification and Isolation of Regulatory B Cells in Mouse and Human

  • Madhvi Menon
  • Elizabeth C. Rosser
  • Claudia MauriEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1899)

Abstract

Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

Key words

Regulatory B cells Inflammation IL-10 T2-MZP B cells Immature B cells CD40 

Notes

Acknowledgments

We would like to thank Dr. Paul Blair for his constructive criticism of the manuscript. Madhvi Menon is funded by a Wellcome Trust PhD studentship (090406/Z/09/Z). Elizabeth C. Rosser was funded by an Arthritis Research UK PhD studentship (MP/19314).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Madhvi Menon
    • 1
  • Elizabeth C. Rosser
    • 1
  • Claudia Mauri
    • 1
    Email author
  1. 1.Centre for Rheumatology, Division of MedicineUniversity College LondonLondonUK

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