Biobanking pp 65-76 | Cite as

Procurement and Storage of Surgical Biospecimens

  • Amin Hojat
  • Bowen Wei
  • Madeline G. Olson
  • Qinwen Mao
  • William H. YongEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1897)


A biobank is an important nexus between clinical and research aspects of pathology. The collection and storage of high quality surgical samples is essential for diagnosis post-surgery, and can also be used to create vaccines, identify therapeutic targets or establish eligibility of cancer patients in a clinical trial. Therefore, personnel handling surgical tissues should follow standard operating procedures (SOP) to maximize efficiency and preserve tissue quality. This chapter is intended to familiarize novice biobank personnel with the issues associated with different steps of surgical tissue collection including patient consent, sample collection, tissue storage, quality control, and distribution.

Key words

Surgical Tissue Biospecimen Biobank Biorepository Procurement Nucleic acids Pathology 



This work was supported in part by NIH:NCI P50-CA211015, NIH:NIMH U24 MH100929, the Art of the Brain Foundation, and the Henry E. Singleton Brain Cancer Research Program.


  1. 1.
    Tuck MK, Chan DW, Chia D et al (2009) Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group. J Proteome Res 8(1):113–117. Scholar
  2. 2.
    Shabikhani M, Lucey GM, Wei B et al (2014) The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank setting. Clin Biochem 47:258–266. Scholar
  3. 3.
    ISBER (2012) Best practices for repositories collection, storage, retrieval, and distribution of biological materials for research international society for biological and environmental repositories. Biopreserv Biobank 10(2):79–161. Scholar
  4. 4.
    Harty-Golder B (2004) Retention and ownership of blocks. MLO Med Lab Obs 36:37PubMedGoogle Scholar
  5. 5.
    Hakimian R, Taube S, Bledsoe M, et al (2004) National Cancer Institute Cancer Diagnosis Program: 50—state survey of laws regarding the collection, storage, and use of human tissue specimens and associated data for research. National Institutes of Health, Bethesda, MD, NIH Publication No: 05-5628Google Scholar
  6. 6.
    Yong WH, Dry SM, Shabikhani M (2014) A practical approach to clinical and research biobanking. In: Day CE (ed) Histopathology, methods and protocols. Humana Press, New YorkGoogle Scholar
  7. 7.
    Kay AB, Estrada DK, Mareninov S et al (2011) Considerations for uniform and accurate biospecimen labeling in a biorepository and research environment. J Clin Pathol 64:634–636. Scholar
  8. 8.
    Lou JJ, Mirsadraei L, Sanchez DE et al (2014) A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories. Clin Biochem 47:267–273. Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Amin Hojat
    • 1
  • Bowen Wei
    • 1
  • Madeline G. Olson
    • 1
  • Qinwen Mao
    • 2
  • William H. Yong
    • 1
    • 3
    • 4
    Email author
  1. 1.Division of Neuropathology, Department of Pathology and Laboratory MedicineDavid Geffen School of Medicine at UCLALos AngelesUSA
  2. 2.Department of Pathology and Laboratory Medicine, Feinberg School of MedicineNorthwestern UniversityChicagoUSA
  3. 3.Brain Tumor Translational ResourceDavid Geffen School of Medicine at UCLALos AngelesUSA
  4. 4.Jonsson Comprehensive Cancer CenterDavid Geffen School of Medicine at UCLALos AngelesUSA

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