Biobanking pp 425-431 | Cite as

Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines

  • Laura Gosa
  • Lisa Ta
  • David A. NathansonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1897)


Patient tumor tissue processing is an important step in the generation of clinically relevant specimens for in vitro and in vivo studies. Proper disassociation and tissue sample cleanup is a multistep, time-consuming process that ultimately effects the generation of patient derived xenografts and neurosphere cultures. Here we describe a detailed protocol on how to process and disassociate patient glioma tissue and subsequent steps on orthotopic implantation and in vitro generation of neurospheres.

Key words

Tissue disassociation Tissue digestion Tumor processing Neurospheres Orthotopic xenografts Patient derived xenografts Brain tumor digestion Enzymatic digestion 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Molecular and Medical PharmacologyDavid Geffen School of Medicine at UCLALos AngelesUSA
  2. 2.Ahmanson Translational Imaging DivisionDavid Geffen School of Medicine at UCLALos AngelesUSA

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