Biobanking pp 313-323 | Cite as

Making a Tissue Microarray

  • Matthew Koo
  • Jill M. Squires
  • Daphne Ying
  • Jiaoti HuangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1897)


Tissue microarray (TMA) is a widely used, high-throughput, cost-effective, and tissue and reagent-conserving method of performing molecular analysis. Multiple donor tissue cores are procured and transferred into a recipient TMA block for simultaneous differential and comparative molecular profiling under theoretically identical performance conditions. Described herein is a discussion of the theory behind the TMA, an overview of the concepts and principles of TMA design and construction, a brief summary of its advantages and disadvantages, and a sample protocol of TMA construction.

Key words

Tissue microarray Immunohistochemistry Molecular profiling Pathology Research Comparative analysis 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Matthew Koo
    • 1
  • Jill M. Squires
    • 1
  • Daphne Ying
    • 1
  • Jiaoti Huang
    • 1
    • 2
    Email author
  1. 1.Department of Pathology and Laboratory MedicineDavid Geffen School of Medicine at UCLALos AngelesUSA
  2. 2.Department of PathologyDuke University School of MedicineDurhamUSA

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