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Biobanking pp 299-311 | Cite as

An Introduction to Performing Immunofluorescence Staining

  • Kyuseok Im
  • Sergey Mareninov
  • M. Fernando Palma Diaz
  • William H. YongEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1897)

Abstract

Immunofluorescence (IF) is an important immunochemical technique that allows for detection and localization of a wide variety of antigens in different types of tissues of various cell preparations. IF allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry, employing various microscopy techniques. There are two methods available, depending on the scope of the experiment or the specific antibodies in use: direct (primary) or indirect (secondary). Here, we describe preparation of specimens preserved in different types of media and step-by-step methods for both direct and indirect immunofluorescence staining.

Key words

Immunofluorescence Immunohistochemistry Immunocytochemistry Fixation Antigen retrieval Fluorescence Fluorophore 

Notes

Acknowledgments

This work was supported in part by NIH:NCI P50-CA211015, NIH:NIMH U24 MH100929, the Art of the Brain Foundation, and the Henry E. Singleton Brain Cancer Research Program.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kyuseok Im
    • 1
    • 2
  • Sergey Mareninov
    • 1
    • 2
  • M. Fernando Palma Diaz
    • 1
  • William H. Yong
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Pathology and Laboratory MedicineDavid Geffen School of Medicine at UCLALos AngelesUSA
  2. 2.Brain Tumor Translational ResourceDavid Geffen School of Medicine at UCLALos AngelesUSA
  3. 3.Jonsson Comprehensive Cancer CenterDavid Geffen School of Medicine at UCLALos AngelesUSA

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