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Biobanking pp 191-212 | Cite as

Procurement and Preservation of Plants

  • Allison D. Rudalevige
  • Jeffrey D. Whitman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1897)

Abstract

Plants have been used by humans for thousands of years for a great variety of purposes. While many people collect plants as a hobby, specimen collections also contribute to the broader scientific community. To provide the greatest value, the specimen must be collected and documented properly. This chapter provides basic guidelines on how to collect plants in the field, process the specimen through pressing and drying, and properly mount the specimen for long-term storage. While the chapter focuses on techniques used for vascular plants, special cases and alternative techniques are also summarized.

Key words

Botany Floristics Vascular plants Plant collecting Curation Herbarium Molecular studies 

Notes

Acknowledgments

We would like to thank the staff of the Rancho Santa Ana Botanic Garden herbarium and library, especially herbarium collections manager, Mare Nazaire, and workroom manager, Rachel Poutasse, for the crash course on what happens to a plant after it is passed on to them. I have a much greater appreciation for the staff and volunteers who deal with the 12,000 to 15,000 specimens deposited each year.

We would also like to thank the California Native Plant Society and the UC and Jepson Herbaria, specifically, Dr. Brent Mishler, David Baxter, and Danny Slakey, for providing a workshop on specimen collection while I was preparing this chapter.

Finally, we would also like to thank Fred Roberts, Jr. and Christine Rudalevige for the technical and language review of the manuscript.

References

  1. 1.
    Forman L, Bridson D (1989) The herbarium handbook. Royal Botanic Gardens, KewGoogle Scholar
  2. 2.
    Smithsonian Institution (2015) Historical expeditions. http://botany.si.edu/colls/expeditions/index.cfm. Accessed 1 Oct 2015
  3. 3.
    Fish L (1999) Preparing herbarium specimens. Strelitzia 7. South African National Botanical Institute, PretoriaGoogle Scholar
  4. 4.
    Funk V (2004) 100 uses for an herbarium: well at least. U.S. National Herbarium, Smithsonian Institution, Washington, DC, p 72Google Scholar
  5. 5.
    The New York Botanic Garden (2007) Index herbarium: a global directory of public herbaria and associated staff. http://sciweb.nybg.org/science2/IndexHerbariorum.asp. Accessed 1 Oct 2015
  6. 6.
    Nazaire M (2015) To collect or not to collect. Paper presented at the conservation conference, Jan 2015. California Native Plant Society, pp 13–17Google Scholar
  7. 7.
    Kaczmarczyk A, Menon A, Al-Hanbali A, Funnekotter B, Bunn E, Phang PY, Mancera RL (2012) Current issues in plant cryopreservation. InTech Open Access Publisher, RijekaCrossRefGoogle Scholar
  8. 8.
    Harris JG, Harris MW (2001) Plant identification terminology: an illustrated glossary., 2nd edn. Spring Lake Pub, PaysonGoogle Scholar
  9. 9.
    Flora of North America Association (2008) Flora of North America. http://floranorthamerica.org/. Accessed 1 Oct 2015
  10. 10.
    Baldwin BG, Goldman DH, Keil DJ et al (2012) The Jepson manual: vascular plants of California., 2nd edn. University of California Press, BerkeleyGoogle Scholar
  11. 11.
    Global Biodiversity Information Facility (2015) GBIF. http://www.gbif.org/. Accessed 1 Oct 2015
  12. 12.
    Integrated Digitized Biocollections (2015) iDigBio Portal. https://www.idigbio.org/. Accessed 1 Oct 2015
  13. 13.
    Jepson Flora Project (eds) (2015) Jepson eFlora. http://ucjeps.berkeley.edu/IJM.html. Accessed 1 Oct 2015
  14. 14.
    Wilson P (ed) (2015) California Moss eFlora. http://ucjeps.berkeley.edu/CA_moss_eflora/. Accessed 1 Oct 2015
  15. 15.
    Consortium of California Herbaria (2015) Consortium of California herbaria data. http://ucjeps.berkeley.edu/consortium/. Accessed 1 Oct 2015
  16. 16.
    SEINet (2015) Taxon search. http://swbiodiversity.org/seinet/index.php. Accessed 1 Oct 2015
  17. 17.
    California Native Plant Society (2015) CNPS. https://www.cnps.org. Accessed 1 Oct 2015
  18. 18.
    Convention on International Trade in Endangered Species of Wild Fauna and Flora (2015) The CITES electronic permitting toolkit. https://cites.org/eng/prog/e/e-permitting-toolkit.php. Accessed 1 Oct 2015
  19. 19.
    U.S. Fish and Wildlife Service (2015) Permits overview. http://www.fws.gov/permits/overview/overview.html. Accessed 1 Oct 2015
  20. 20.
    California Department of Fish and Wildlife (2015) Permits for threatened and endangered plant species. https://www.wildlife.ca.gov/Conservation/Plants/Permits. Accessed 1 Oct 2015
  21. 21.
    California Native Plant Society (2015) Final draft California Native Plant Society terms and conditions guidelines for botanical collecting. Presented at the California Native Plant Society and University of California Jepson specimen collection workshop, Rancho Santa Ana Botanic Garden, 9 Oct 2015Google Scholar
  22. 22.
    Weising K, Nybom H, Wolff K, Kahl G (2005) DNA fingerprinting in plants: principles, methods, and applications. CRC Press, Boca RotanCrossRefGoogle Scholar
  23. 23.
    Pyle MM, Adams RP (1989) In situ preservation of DNA in plant specimens. Taxon 38:576–558CrossRefGoogle Scholar
  24. 24.
    Doyle JJ, Dickson EE (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715–722CrossRefGoogle Scholar
  25. 25.
    Rogstad SH (1992) Saturated NaCl-CTAB solution as a means of field preservation of leaves for DNA analyses. Taxon 41:701–708CrossRefGoogle Scholar
  26. 26.
    Murray MG, Pitas JW (1996) Plant DNA from alcohol-preserved samples. Plant Mol Biol Rep 14:261–265CrossRefGoogle Scholar
  27. 27.
    Kami D (2012) Cryopreservation of plant genetic resources. In: Katkov I (ed) Current frontiers in cryobiology. InTech, RijekaGoogle Scholar
  28. 28.
    MacFarlane RB (1985) Collecting and preserving plants for science and pleasure. Arco Publishing, Inc., New YorkGoogle Scholar
  29. 29.
    Ross TS (1996) Herbarium specimens as documents: purposes and general collecting techniques. Crossosoma 22(1):3–39Google Scholar
  30. 30.
    Brayshaw TC (1996) Plant collecting for the amateur. Royal British Columbia Museum, Victoria, British ColumbiaGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.PsomasSanta AnaUSA
  2. 2.Departments of Pathology and Laboratory MedicineUniversity of California, San FranciscoSan FranciscoUSA

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