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Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry

  • Christopher D. WileyEmail author
  • Sonnet Davis
  • Arvind Ramanathan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1896)

Abstract

Senescent cells display altered metabolic signatures that can either be causal for aspects the senescent phenotype, or act as biomarkers for senescence. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.

Key words

Senescence Mass spectrometry Metabolomics Metabolites 

References

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    Bajad SU et al (2006) Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry. J Chromatogr A 1125(1):76–88CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Christopher D. Wiley
    • 1
    Email author
  • Sonnet Davis
    • 1
  • Arvind Ramanathan
    • 1
  1. 1.Buck Institute for Research on AgingNovatoUSA

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