Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells

  • Nathan M. Kingston
  • Andrew M. Tilston-Lunel
  • Julia Hicks-Berthet
  • Xaralabos VarelasEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)


The transcriptional coactivator with PDZ-binding motif (TAZ), which is encoded by the WWTR1 gene, is a key transcriptional effector of the Hippo signaling pathway. TAZ function has been implicated in a variety of developmental processes and diseases, most notably in driving oncogenesis. Given that nuclear-cytoplasmic localization dynamics dictate TAZ activity, techniques for visualizing TAZ localization are critical for its study. Here we describe an immunofluorescence microscopy protocol that allows for the visualization of TAZ subcellular localization in mammalian cells, offering an approach that can aid in the analysis of TAZ regulation and function.

Key words

TAZ WWTR1 Hippo pathway Immunofluorescence microscopy 



We would like to thank Dr. Jeffrey Wrana (Lunenfeld-Tanenbaum Research Institute, Toronto, Canada) for providing the Taz-loxP/loxP mice. X.V. is supported by NIH R01HL124392, the March of Dimes Foundation Grant no. 1-FY17-375, and an American Cancer Society Ellison New England Research Scholar Grant (RSG-17-138-01-CSM). N. M. K. is supported by NIH T32HL007035-40. J.H.B. is supported by NIH F31HL13250601.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nathan M. Kingston
    • 1
  • Andrew M. Tilston-Lunel
    • 1
  • Julia Hicks-Berthet
    • 1
  • Xaralabos Varelas
    • 1
    Email author
  1. 1.Department of BiochemistryBoston University School of MedicineBostonUSA

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