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Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs

  • Jiajie Xu
  • Ting Su
  • Sherzod A. Tokamov
  • Richard G. Fehon
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)

Abstract

Examining the subcellular localization of Hippo pathway components has helped elucidate the molecular mechanisms that regulate the pathway. Here we describe methods for performing live imaging of fluorescently tagged Hippo pathway components in Drosophila wing imaginal discs.

Key words

Hippo pathway components Live imaging Drosophila wing imaginal discs 

Notes

Acknowledgments

T.S. and J.X. were recipients of the Children’s Tumor Foundation Young Investigator Award (2012-01-033, 2013-01-020, and 2014-01-020, respectively), and S.T. was supported by NIH training grant T32 GM007183. This work was supported by a grant from the NIH to R.G.F. (NS034783).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jiajie Xu
    • 1
    • 2
  • Ting Su
    • 1
  • Sherzod A. Tokamov
    • 1
    • 2
  • Richard G. Fehon
    • 1
    • 2
  1. 1.Department of Molecular Genetics and Cell BiologyThe University of ChicagoChicagoUSA
  2. 2.Committee on Development, Regeneration and Stem Cell BiologyThe University of ChicagoChicagoUSA

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