Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs
Examining the subcellular localization of Hippo pathway components has helped elucidate the molecular mechanisms that regulate the pathway. Here we describe methods for performing live imaging of fluorescently tagged Hippo pathway components in Drosophila wing imaginal discs.
Key wordsHippo pathway components Live imaging Drosophila wing imaginal discs
T.S. and J.X. were recipients of the Children’s Tumor Foundation Young Investigator Award (2012-01-033, 2013-01-020, and 2014-01-020, respectively), and S.T. was supported by NIH training grant T32 GM007183. This work was supported by a grant from the NIH to R.G.F. (NS034783).