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Measuring the Kinase Activities of the LATS/NDR Protein Kinases

  • Alexander HergovichEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)

Abstract

The Hippo tissue growth control and regeneration pathway is a main regulator of the YAP/TAZ effectors. In this regard, the LATS/NDR serine/threonine protein kinases can function as central components of the Hippo core module. More specifically, LATS/NDR-mediated phosphorylation of YAP/TAZ on different residues can regulate the subcellular localization and/or stability of YAP/TAZ. Therefore, the assessment of LATS/NDR activities can serve as readout for the activity status of the Hippo pathway. Here, we describe our preferred methodology regarding the measurement of the activities of LATS/NDR kinases.

Key words

Hippo pathway Protein kinases Kinase assays Kinase substrates LATS1 LATS2 NDR1 NDR2 STK38 STK38L 

Notes

Acknowledgments

The Hergovich laboratory was supported by the Wellcome Trust (090090/Z/09/Z), BBSRC (BB/I021248/1), Worldwide Cancer Research (AICR; 11-0634), UCL Cancer Research UK Centre, and the National Institute for Health Research University College London Hospitals Biomedical Research Centre.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Cancer InstituteUniversity College LondonLondonUK

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