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GST Pull-Down Assay to Measure Complex Formations

  • Sun-Yong Kim
  • Toshio HakoshimaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)

Abstract

The GST pull-down assay is an intuitive and fast in vitro method for analyzing protein–protein or protein–ligand interactions and is comprised of a “bait” which is a GST-fused protein expressed in E. coli host or a baculovirus expression system and a “prey” which comprises putative binding partner protein(s) or other ligand molecule(s). This method is suitable for examining the direct interaction between two purified proteins and estimating the extent of the affinity.

Key words

GST pull-down assay Glutathione S-transferase Glutathione-conjugated resin Direct protein–protein interaction In vitro assay Screening of binding partners 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Structural Biology LaboratoryNara Institute of Science and TechnologyNaraJapan

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