Isolation of Lactic Acid Bacteria Eliminating Trimethylamine (TMA) for Application to Fishery Processing

  • Satoshi Mohri
  • Makoto KanauchiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1887)


Fishy odor of fish flesh (meat) presents a severe problem for marine production. The main cause of fishy odor is trimethylamine (TMA), which increases during storage. It is produced from trimethylamine oxide (TMAO), an osmosis-regulating substance in fish cells that functions by a reduction reaction. Bacterial growth in fish meat increases TMA. Its odor reduces the commercial value of the meat. Technologies for its regulation and elimination are desired. This chapter presents a description of the use of lactic acid to eliminate TMA. The lactic acid is producible safely by bacteria during food processing using picric acid—toluene.

A method of eliminating TMA was demonstrated using Lactobacillus plantarum H78. Furthermore, an assay method was explained for reducing TMA in fish meat by fermenting the H78 strain.

Key words

Fish meat Lactobacillus plantarum Picric acid–toluene method Spectrophotometry Trimethylamine 


  1. 1.
    Ministry of Agriculture, Forestry and Fisheries. Accessed 20 Jan 2018
  2. 2.
    Koizumi C, Ohashi T (2005) Processing and storage of marine food products. Kouseisha Kouseikaku Co., Ltd, TokyoGoogle Scholar
  3. 3.
    Kimura M, Takeuchi K, Nozawa H et al (2006) The mechanism and oxygen gas inhibition of the degradation of trimethylamine-N-oxide in walleye pollack flesh during storage. J Japan Soc Fish Sci 72(5):911–917 in JapaneseCrossRefGoogle Scholar
  4. 4.
    Yamada K (1968) Post-mortem breakdown of trimethylamine oxide in fish and marine invertebrates. J Japan Soc Fish Sci 34(6):541–551 in JapaneseCrossRefGoogle Scholar
  5. 5.
    Charalambous G (2013) Off-flavors in foods and beverages. Elsevier Science, Amsterdam, p 154Google Scholar
  6. 6.
    Yamamoto K, Kato T (2007) Development of unsalted fish sauce. Using antibacterial lactic acid bacteria. Annu Rep Food Res Inst 6:114–117 (in Japanese)Google Scholar
  7. 7.
    Fujii T (1978) Microbial Flora of “Kusaya” Brine of Niijima and Oshima Islands. J Japan Soc Fish Sci 44(1):45–48 in JapaneseCrossRefGoogle Scholar
  8. 8.
    Fujii T (1975) Removal of fishy odors by microorganisms. Koryo 113:46–48Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Food ManagementMiyagi UniversitySendaiJapan

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