Proteomic Profiling for Target Identification of Biologically Active Small Molecules Using 2D DIGE
Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. This system compares the similarities in protein expression of 296 spots detected in the gel among the test compounds.
Key wordsProteomics Two-dimensional florescent electrophoresis (2D DIGE) Profiling Target identification of compounds Multivariate analysis
We thank Ms. H. Kondo, Ms. K. Noda, Ms. Y. Nakata, Ms. Y. Hirata, and Ms. M. Tanaka for conducting proteomic analysis. This work was supported in part by JSPS KAKENHI Grant Numbers JP16H06276, JP17H06412, JP18H03945, JP17K07783, AMED under Grant Number JP18cm0106112 and the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovative technology).
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