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Autophagy pp 621-642 | Cite as

Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

  • Thomas G. McWilliams
  • Ian G. Ganley
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.

Key words

Mitophagy Autophagy Mitochondria Mouse models Neurodegeneration Metabolism Cancer Immunology Cardiology Vascular biology Nephrology Developmental biology Histology Microscopy 

Notes

Acknowledgments

We gratefully acknowledge the outstanding support from our colleagues and collaborators who have enabled us to develop and refine our methods using mito-QC. In particular, we wish to thank Dr. Alan R. Prescott for his expertise in microscopy and Dr. Graeme Ball for his expertise in image analysis. We thank Dr. Ngaire Dennison for her guidance on animal experiments. We thank Lambert Montava-Garriga, Dr. Jin-Feng Zhao, and Dr. François Singh for their critical reading of the manuscript. This work was funded by Medical Research Council, UK (IGG; MC_UU_12016/4).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life SciencesUniversity of DundeeDundeeUK
  2. 2.Translational Stem Cell Biology Research ProgramResearch Programs Unit, Faculty of Medicine, University of HelsinkiHelsinkiFinland

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