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Autophagy pp 589-600 | Cite as

Investigating Non-selective Autophagy in Drosophila

  • Szabolcs Takáts
  • Sarolta Tóth
  • Győző Szenci
  • Gábor JuhászEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

Drosophila melanogaster is a popular model organism in molecular genetics and cell biology. Various Drosophila tissues have been successfully used for studying autophagy, and our knowledge about the genetic regulation of this process is constantly growing. It is important to use assays that distinguish between non-selective autophagy and the selective forms. Here we introduce a selection of proven methods, which, taking into account their limitations, are suitable to measure non-selective autophagy in Drosophila fat and other tissues.

Key words

Drosophila Non-selective autophagy Fluorescent dyes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Szabolcs Takáts
    • 1
    • 2
  • Sarolta Tóth
    • 2
  • Győző Szenci
    • 2
  • Gábor Juhász
    • 2
    • 3
    Email author
  1. 1.Hungarian Academy of Sciences, Premium Postdoctorate Research ProgramBudapestHungary
  2. 2.Department of Anatomy, Cell and Developmental BiologyEötvös Loránd UniversityBudapestHungary
  3. 3.Institute of GeneticsBiological Research CentreSzegedHungary

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