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Autophagy pp 447-454 | Cite as

Methods for the Study of Entotic Cell Death

  • Jens C. Hamann
  • Sung Eun Kim
  • Michael Overholtzer
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.

Key words

Entosis Cell-in-cell Cannibalism Engulfment Time-lapse Soft agar Anoikis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jens C. Hamann
    • 4
    • 1
    • 2
  • Sung Eun Kim
    • 3
    • 1
  • Michael Overholtzer
    • 1
  1. 1.Cell Biology ProgramSloan Kettering Institute for Cancer ResearchNew YorkUSA
  2. 2.Louis V. Gerstner, Jr. Graduate School of Biomedical SciencesMemorial Sloan Kettering Cancer CenterNew YorkUSA
  3. 3.Helen Diller Family Comprehensive Cancer CenterUniversity of CaliforniaSan FranciscoUSA
  4. 4.Meyer Cancer CenterWeill Cornell MedicineNew YorkUSA

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