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Autophagy pp 429-445 | Cite as

Automated Detection of Autophagy Response Using Single Cell-Based Microscopy Assays

  • Amelie J. Mueller
  • Tassula Proikas-Cezanne
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

The fluorescence microscopy-based detection of intracellular LC3, p62, and/or WIPI punctate structures is a robust tool to monitor and assess macroautophagy/autophagy in single cells. This method was established for automated high-throughput/content analysis to reliably detect narrow differences in autophagy activity/capacity and to provide screening opportunities for biological and chemical libraries.

Key words

Autophagy Automated high-throughput analysis LC3 p62 WIPI 

Notes

Acknowledgments

We apologize to researchers whose work we were unable to cite due to length constraints. Amelie J. Mueller received a predoctoral stipend from the International Max Planck Research School “From Molecules to Organisms.” Tassula Proikas-Cezanne receives grant support from the Deutsche Forschungsgemeinschaft (DFG), SFB/TRR 209 (project B02), and FOR 2625 (project 1).

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Interfaculty Institute of Cell BiologyEberhard Karls University TuebingenTübingenGermany
  2. 2.International Max Planck Research School “From Molecules to Organisms”TübingenGermany

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