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Autophagy pp 375-387 | Cite as

Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-Throughput Screening

  • Martina Wirth
  • Sharon A. ToozeEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

Autophagy is an evolutionarily conserved degradative pathway, and the core autophagy machinery acts in a highly regulated, hierarchical manner to engulf cytoplasmic material in a double-membrane-bound organelle and deliver it to the lysosome. High-throughput screening approaches lead to the identification of novel autophagy regulators, and we describe an autophagy pathway mapping strategy to determine the stage of the autophagy pathway at which these novel candidate proteins function.

Key words

Autophagy pathway mapping mTORC1 ULK complex WIPI2 ATG9 LC3 Autophagosome maturation Autophagic flux 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Molecular Cell Biology of Autophagy LaboratoryThe Francis Crick InstituteLondonUK

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