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X-Ray Absorption Spectroscopy of Metalloproteins

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Metalloproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1876))

Abstract

X-ray absorption spectroscopy (XAS) has been widely used as a powerful local structural tool for studying the metal binding sites in metalloproteins, owing to its element specificity, chemical sensitivity, and minimum requirement for sample preparation. The principle subject of this chapter is to provide a general introduction of this technique to molecular biologists and biochemists, with the intention to fill the knowledge gaps in interdisciplinary communication and collaboration. An update of the XAS-based technique applied recently to metalloproteins is also briefly introduced at the end of the chapter.

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Acknowledgments

The data presented in this chapter were collected at the Stanford Synchrotron Radiation Lightsource and Canadian Light Source. EXAFSPAK and IFFEFIT were used for analyzing data and generating figures in this chapter. The author thanks Dr. De-Tong Jiang at the Guelph University and Dr. Graham George at the University of Saskatchewan for invaluable discussions and contributions to the figures.

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA

    Limei Zhang

  2. Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE, USA

    Limei Zhang

  3. Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE, USA

    Limei Zhang

Authors
  1. Limei Zhang
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Correspondence to Limei Zhang .

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Editors and Affiliations

  1. Department of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA, USA

    Yilin Hu

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Zhang, L. (2019). X-Ray Absorption Spectroscopy of Metalloproteins. In: Hu, Y. (eds) Metalloproteins. Methods in Molecular Biology, vol 1876. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8864-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8864-8_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8863-1

  • Online ISBN: 978-1-4939-8864-8

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