Methods to Probe Calcium Regulation by BCL-2 Family Members

  • Marcos A. Carpio
  • Samuel G. KatzEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1877)


BCL-2 family members have additional roles beyond direct regulation of mitochondrial outer membrane permeabilization (MOMP) in apoptosis. One such important function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.

Key words

Apoptosis Calcium BCL-2 Family Mitochondria Endoplasmic reticulum 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of PathologyYale University School of MedicineNew HavenUSA
  2. 2.CIQUIBIC-Department of Biological ChemistryNational University of CordobaCordobaArgentina

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