Measurement of Buprenorphine and Norbuprenorphine in Urine

  • Andrea R. Terrell
  • Vipin Adhlakha
  • Poluru Reddy
Part of the Methods in Molecular Biology book series (MIMB, volume 1872)


Buprenorphine, a synthetic opioid possessing both analgesic and opioid receptor antagonist properties, has proven to be an effective therapeutic aid for opioid dependency and chronic pain management. The downside, as with all opioids, natural or synthetic, is its potential for misuse and abuse. The euphoria induced by buprenorphine leads to abuse. Additionally, individuals with an active addiction to short-acting opioids such as heroin may use buprenorphine between doses of their drug of choice to stave off withdrawal symptoms. As such, buprenorphine monitoring is utilized in medication-assisted therapy programs for opioid dependency, as well as chronic pain management settings. Buprenorphine may also be included in drug testing programs for law enforcement purposes. The assay described here was designed to detect and quantify both buprenorphine and its metabolite norbuprenorphine.

Key words

Buprenorphine Norbuprenorphine LC-MS/MS Opioid 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Andrea R. Terrell
    • 1
  • Vipin Adhlakha
    • 2
  • Poluru Reddy
    • 2
  1. 1.Phoenix LaboratoriesIndianapolisUSA
  2. 2.Aria DiagnosticsIndianapolisUSA

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